FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962709
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34800.11746006779606098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA21450.042.1278151
ACACTCT34350.040.7858771
GCCACAC20300.026.0529821
CACTCTT86500.019.233311
ACTCTTT87500.018.8054182
GCACCCA11350.016.9724041
ATGCCGG10500.016.33280270
CCTATGC10400.015.8215984
AGGCCGG3108.8402885E-1015.80593768
GCACCAC19250.015.4655271
GTGCCGG4350.015.28685970
GGCCGGC2858.5543434E-814.73636269
GCCGGCC2858.5543434E-814.73636270
TCTTTCC111750.014.5363824
CTTTCCC103850.014.5296995
CTCTTTC114700.014.4066983
CCCTACA77400.014.3341599
GGGCGCG2001.7556874E-413.99954466
TTCCCTA96950.013.9345647
GACCGCG1550.00737961913.54794668
Produced by FastQC (version 0.11.5)