FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5310
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGT1222.297551789077213TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGG811.5254237288135595TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGTT340.64030131826742TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGTT130.2448210922787194TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTGGGGT80.15065913370998116TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT70.1318267419962335TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGTG60.11299435028248588TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGG60.11299435028248588TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGCT60.11299435028248588TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATCG500.070.0000139
ATCGGGG453.6379788E-1270.0000142
GAATCGG453.6379788E-1270.0000140
AATCGGG453.6379788E-1270.0000141
TTGGGGG150.00210437570.050
TCAGAAT600.070.037
CAGAATC600.070.038
CAGTCAC700.065.027
CCAGTCA700.065.026
TGTCAGA700.065.035
TCCAGTC700.065.025
AACTCCA700.065.022
ATGTCAG700.065.034
TCACATG700.065.030
GTCAGAA700.065.036
AGTCACA700.065.028
CATGTCA700.065.033
TCGGGGG452.746674E-1062.22222543
GTCTGAA800.061.2517
GTCACAT750.060.6666729
Produced by FastQC (version 0.11.5)