FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592344
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC52600.2029051699928713TruSeq Adapter, Index 14 (100% over 38bp)
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCG50340.19418719120610536TruSeq Adapter, Index 14 (100% over 40bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT46790.1804930209879553TruSeq Adapter, Index 8 (97% over 37bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCC46410.17902716614770262TruSeq Adapter, Index 14 (100% over 39bp)
ACACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT37300.1438852251090133TruSeq Adapter, Index 8 (97% over 37bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC35930.13860043265862865Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG34820.1343185935199958Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT32280.12452051116672788TruSeq Adapter, Index 8 (97% over 36bp)
ATGAGAAATGGGTGAATGTTGAGATAATTGTTGGGATTCCATTGTTGATA31530.12162737661359757No Hit
CACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT30710.1184642161688418TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29790.1149153044503353No Hit
CTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC29350.11321799884583218TruSeq Adapter, Index 14 (100% over 38bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA26510.10226266267131215Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT64900.055.1970631
CGCTTGC3850.038.18100723
GCCCACA14650.035.6150631
GGCGGTA4600.033.4775542
GGCGATT4250.032.9404854
AGTGGCG4800.032.08265339
CGGTAGT4750.031.68353844
CACTCTT147700.031.1055871
GCGCTTG4800.030.6243522
TGGCGGT5200.030.28781941
AGTCCAG4900.029.99936516
CAGGCTT5450.028.9040455
GAGCTGC5500.028.63575632
GCTTGCA5650.028.49497224
GCGGTAG5350.028.13024543
GCCACTA5050.027.72218339
CGAGCCT1900.027.63099353
CCAGAGG6050.027.18950522
TGGCGAT5050.027.02912953
AGGCTTG5750.026.7872076
Produced by FastQC (version 0.11.5)