FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702649
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT23750.035.0975651
GCGTGAA17750.029.5764268
GCGTGGA10300.028.5426868
GCCCACA16300.028.3628561
GGGCGTG6700.027.68558966
TGCGTGA12100.026.6106367
GGCGTGA8050.026.08603567
CGTGAAA20900.025.95602269
AGTGGCG5750.025.56431639
CTGCGTG12700.025.07785466
CTGCTTG25300.024.90030766
TCGGCGT11450.024.7589565
GCGGTAG5700.024.56053543
CGGTAGT5850.024.52904944
GTGCGTG4300.023.60381766
GTGGCGG6400.023.51479340
GCTTGAA32700.023.4395468
TGGGCGT8450.023.19444865
GTGGGCG7600.023.02550364
GGCGGTA5950.022.94036742
Produced by FastQC (version 0.11.5)