FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8374785
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT54850.038.819951
GGGTGGA62050.033.56068866
GGAAAAA205350.029.72401270
GCGTGGA30200.029.20446666
GTGGAAA107500.028.32477668
GGTGGAA78050.027.62253267
CACTCTT142750.026.5693151
CCGGGGT39150.026.19337857
CGGAGTC10750.026.045777
GAGATCG148700.025.6379956
CGGGGTG118700.025.6226958
ACAGGCT15800.025.4805014
ACGAGTG10500.025.33276438
GCGGGGT90350.025.25661357
GGGCGTG26000.025.1723664
TGCCGGG50200.024.40169555
GCGGTAG15400.024.31748843
TTGGCGG63150.024.16454
CGGACAG7550.024.10527248
GTGGGGG154850.023.91278362
Produced by FastQC (version 0.11.5)