FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8923221
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT102150.044.157771
CACTCTT272400.025.2758661
ACTCTTT303450.022.7052542
GGGATTC44700.021.06198533
GGCTATA44350.020.36013453
GTTGGGA46150.019.9452130
GTACGCG5100.019.9013421
AGGCTAT47750.018.91040852
TGTTGGG49500.018.87820229
TAAAGGC49050.018.55192249
AAGGCTA51150.017.6534151
GGTATAC51550.017.44853666
GCTATAA52100.017.39869154
GTGAATG44400.017.34180312
ACGCGCC5900.017.20285623
CTTTCCC377200.017.1985635
TGGGATT56100.017.1563332
CTCTTTC405250.016.9670723
TCTTTCC401000.016.928634
TACGCGC6150.016.50355322
Produced by FastQC (version 0.11.5)