FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8800707
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT129400.045.7972031
GGGTGGA35100.025.7256966
GCGTGGA37650.025.19177666
CACTCTT343350.024.6758251
GCCCACA27400.023.9036331
ACTCTTT376750.022.7571742
GTGGAAA85600.022.36495468
CGTGGAA47350.020.47454567
GCGTGAA38000.020.35462666
GGAAAAA160450.019.67529570
TGGGGGT45650.019.39698463
TCGGCGT34700.018.86108263
GGGGGGA20300.018.79251566
GGTGGAA51350.018.33438567
GGGGGTG49200.018.13967764
GGGCGTG27350.017.78737864
CTCTTTC487550.017.628393
CTTTCCC467400.017.5569575
GTGGGGG59250.017.42561562
CGTGAAA45800.017.42303767
Produced by FastQC (version 0.11.5)