FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10123588
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2923312.8876224516446145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA16450.033.2077751
CACTCTT97200.031.1624031
ACACTCT72850.025.8603151
GGAAAAA67100.021.22481770
GCGAGAT10400.021.2035244
ACTCTTT158400.019.666892
GCCACAC17150.019.601451
CCCTACA128900.019.0568899
CGCGAGA6300.018.8903143
TGAGATC21950.018.6574465
TCCCTAC136050.018.6469278
GGGGAAA34950.018.62258768
GGGGGAA30300.018.59334467
TTCCCTA148100.018.3347267
CCTACAC133150.018.13352210
GGGGGGA31350.017.970666
GGTGGGA9400.017.8684866
GAGATCG75050.017.5829376
GGGATTC10000.017.51291533
AAAGATC32850.017.4746825
Produced by FastQC (version 0.11.5)