FastQCFastQC Report
Tue 12 Apr 2022
HKTHMAFX3_n01_as3-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTHMAFX3_n01_as3-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6517418
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT189200.2902990110500815Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC161170.24729118187601287Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT75410.11570532993280469TruSeq Adapter, Index 16 (100% over 37bp)
CTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC72580.11136311956667502Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC72060.11056525759127311Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC71220.10927640363100846TruSeq Adapter, Index 16 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)