FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13620340
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156630711.499764323063888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA8100.023.2826278
ACCCGCG9150.020.5768975
TGACCCG9300.019.8156243
GACCCGC9600.019.211924
GCGTTAG9600.018.9257289
AGCAGTG45950.018.6138972
GAGTACT39000.018.3696220
CCCGCGT10250.017.688656
AGAGTAC48400.017.56095719
CGCAGAG50900.016.97393616
CCCGGAT7500.016.7711059
GTATCAA54050.015.8376539
CAGAGTA55100.015.80822418
ATCAACG56250.015.4460411
AGTAGTC8550.015.046082
AGTACTT47250.014.84123921
TACCCGG8250.014.8059957
CGCCGTA7100.014.79975247
ACCCGGA8850.014.2063478
GTACTTT50750.013.94864522
Produced by FastQC (version 0.11.5)