FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n02_Tyr_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n02_Tyr_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27224430
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2564760.942080330056497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC174800.016.53877318
CGCAGAG178400.016.28350615
GTACGGG68150.016.02321
GCAGTGG198250.015.1533232
GGTATCA198850.014.8902047
AGTACGG73450.014.86714220
GTGGTAT200800.014.6936915
AGTGGTA203100.014.6833334
GAGTACT130150.014.54848620
TCAACGC198650.014.482326511
AGCAGTG209450.014.4037881
GAGTACG74950.014.3828119
TACGGGG48000.014.36415622
GCAGAGT214450.013.69306316
AACGCAG211450.013.63875113
ACGCAGA212950.013.62515814
CAGAGTA216850.013.49309317
TGGTATC221400.013.4052746
CAACGCA231050.012.7241412
ATCAACG234950.012.45334210
Produced by FastQC (version 0.11.5)