FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n02_Tyr_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n02_Tyr_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30083188
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1263220.41990895379838067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT138550.014.17164220
CGCAGAG195550.013.0297915
AGAGTAC197650.012.92676618
GCAGTGG206700.012.704152
AGTGGTA208700.012.5783684
GTGGTAT211050.012.4211075
AGCAGTG220850.011.9944031
GTACGGG86900.011.96175121
TACGGGG59250.011.93215622
GAGTACG89600.011.91394719
AGTACGG87750.011.80615320
TCAACGC218300.011.79998511
GGTATCA226200.011.7128357
AACGCAG221100.011.58721213
ACGCAGA224800.011.3655114
TGGTATC242300.011.0790416
CAGAGTA241200.010.70885117
GCAGAGT243400.010.61205916
GTATCAA256150.010.3842688
CAACGCA252500.010.17398912
Produced by FastQC (version 0.11.5)