FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n02_Tyr_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n02_Tyr_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27307660
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1526050.5588358724255392No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293630.10752660608781565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGG205850.014.9679322
GAGTACT159500.014.17535620
AGCAGTG220700.014.09843351
GTGGTAT223550.013.6054485
AGTGGTA228300.013.3843344
CGCAGAG230250.013.04216915
AGAGTAC233650.012.9422618
GGTATCA238350.012.7750647
GTACGGG93150.012.73716121
AGTACGG96800.012.32937820
TCAACGC249650.012.084611
GAGTACG99200.011.99580519
TGGTATC261150.011.7670236
AACGCAG260850.011.6059613
ACGCAGA264950.011.4133214
TACGGGG65650.011.30204322
CAGTGGT275950.011.2772783
CAGAGTA275100.011.18305417
GTATCAA273700.011.1506218
GCAGAGT274450.011.14577816
Produced by FastQC (version 0.11.5)