FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n02_Rnf149_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n02_Rnf149_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26109932
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2436390.9331276695779982No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT261740.10024537788914961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGG181150.015.2312212
CGCAGAG186100.014.40617715
AGTGGTA190750.014.3861184
GTGGTAT192500.014.272675
AGAGTAC185750.014.26373918
GAGTACT131150.014.19742820
GTACGGG75000.013.99956921
GGTATCA196050.013.9603487
AGCAGTG203850.013.8213111
AGTACGG79950.013.39581920
TCAACGC203050.013.20321311
TACGGGG53300.012.87012322
ACGCAGA216100.012.6167714
TGGTATC217800.012.6144916
GAGTACG84650.012.610719
AACGCAG214800.012.546144513
CAGAGTA220600.012.24836417
GCAGAGT225300.012.02391916
CAGTGGT234700.011.9468643
GTATCAA233800.011.7511138
Produced by FastQC (version 0.11.5)