FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n02_Rnf149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n02_Rnf149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29673456
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2378520.8015648733332579No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT319840.10778656857495802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT152850.015.18133120
GCAGTGG219500.014.4041772
AGCAGTG231000.013.8032381
AGAGTAC223100.013.78957918
CGCAGAG223500.013.73358115
AGTGGTA236250.013.4083194
GTACGGG88600.013.39127321
GTGGTAT236900.013.3855395
TACGGGG64650.013.3174722
GGTATCA239700.013.0827877
AGTACGG95300.012.78050620
TCAACGC250150.012.28428511
GAGTACG101250.012.09858919
ACGCAGA259100.012.04920714
AACGCAG259000.011.93207613
GCAGAGT262250.011.91784316
TGGTATC267600.011.7842276
CAGAGTA265600.011.76752617
GTATCAA275650.011.3638278
CAACGCA277200.011.31279612
Produced by FastQC (version 0.11.5)