FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n02_Rnf149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n02_Rnf149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26390617
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2495350.9455443955705923No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT335690.12720051221235185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGG193750.016.9290873
AGCAGTG200200.016.4054132
AGTGGTA210150.015.5553245
GTGGTAT215600.015.1619686
GAGTACT174100.014.4944120
GGTATCA230250.014.1363838
CGCAGAG238600.013.28990316
TGGTATC249500.013.1720247
AGAGTAC241550.013.15657519
CAGTGGT253200.013.063224
TCAACGC250950.012.70533712
GTATCAA266200.012.2667019
AACGCAG265200.012.11527314
ACGCAGA266850.011.94855115
GCAGAGT271650.011.85337817
CAGAGTA272250.011.67298718
CAACGCA278950.011.49275413
ATCAACG285000.011.43298911
AGTACTT229450.011.07397621
GTACTTT250350.010.303246522
Produced by FastQC (version 0.11.5)