FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13620340
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277230.20354117444938966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144760.1062822220296997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT38100.015.47651820
CGCAGAG51950.014.30416416
AGCAGTG54650.014.2266412
AGAGTAC53700.013.70838119
CAGAGTA62700.011.85217518
AGTACGG21600.011.79181420
GAGTACG23300.011.37982719
ATCAACG67750.011.37104711
GTATCAA67750.011.2161139
AGTACTT53400.011.17348621
GAGACCG13150.011.16760431
CCGAGAC12250.010.84460229
CGTCTGA12250.010.53741416
GTACTTT58650.010.47076722
GCAGTGG78950.010.0684083
ACGTCTG13750.09.89020615
AGTGGTA85600.09.3676825
CGAGACC15700.09.13064630
GTGGTAT87550.08.9599276
CACGTCT15200.08.7160514
Produced by FastQC (version 0.11.5)