FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n01_Tyr_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n01_Tyr_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27224430
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT277170.10180929407888431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC184850.016.22622718
CGCAGAG186050.016.08394815
GAGTACT133400.015.8728820
GCAGTGG200900.015.4914792
GTACGGG73800.015.31800921
GGTATCA207400.014.7827877
AGTGGTA210700.014.7540054
GTGGTAT211300.014.6955435
TACGGGG49900.014.5887822
AGTACGG77500.014.54153920
TCAACGC211950.014.49830711
AGCAGTG217950.014.3007041
GAGTACG79150.014.23839919
AACGCAG221650.013.737513
CAGAGTA224400.013.60034217
ACGCAGA224550.013.59125614
GCAGAGT228400.013.34683316
TGGTATC235600.013.2689846
GTATCAA239700.012.8198428
ATCAACG246200.012.41030210
Produced by FastQC (version 0.11.5)