FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n01_Tyr_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n01_Tyr_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30083188
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT144100.014.76706620
CGCAGAG200100.013.13552716
GCAGTGG208350.013.09001352
AGAGTAC206150.012.91980519
GTGGTAT212400.012.8564496
AGTGGTA211800.012.7771645
AGCAGTG227250.012.005411
TCAACGC224000.011.78088712
GTACGGG84700.011.73517421
GGTATCA230850.011.7194467
TACGGGG57700.011.52477522
AACGCAG229400.011.48831113
ACGCAGA232550.011.45309615
AGTACGG87050.011.29775520
GAGTACG90200.011.01961419
CAGAGTA249150.010.95691518
TGGTATC247650.010.89926056
AGTACTT205800.010.62892321
GCAGAGT252750.010.59314417
GTATCAA258700.010.51186858
Produced by FastQC (version 0.11.5)