FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n01_Tyr_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n01_Tyr_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27307660
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323360.11841366122179638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGG210400.015.2248432
GAGTACT167200.015.1132120
AGCAGTG230300.014.030892
GTGGTAT232800.013.7295836
AGTGGTA238300.013.398015
AGAGTAC237000.013.18739218
CGCAGAG237400.013.12094515
GGTATCA244550.012.9378027
TACGGGG63250.012.726912522
GTACGGG94400.012.71680321
AGTACGG98850.012.35675920
TCAACGC257550.012.25749111
GAGTACG100050.012.13858819
TGGTATC269600.011.9174287
AACGCAG268850.011.78133214
ACGCAGA269650.011.6944615
AGTACTT223000.011.47277121
CAGTGGT285900.011.461233
CAGAGTA277750.011.36601717
GTATCAA282550.011.247278
Produced by FastQC (version 0.11.5)