FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n01_Rnf149_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n01_Rnf149_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26109932
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT291760.11174291836531784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT133150.016.71745520
GCAGTGG192350.014.3787853
GTACGGG72800.014.13416521
AGAGTAC190700.013.89310719
AGTGGTA197400.013.8867925
CGCAGAG193050.013.76024616
GTGGTAT198250.013.7566146
GGTATCA203450.013.3148868
TACGGGG52600.013.30756122
AGCAGTG210150.013.2778792
AGTACGG79650.013.00649120
GAGTACG80000.012.86209119
TCAACGC210900.012.82794712
AGTACTT184650.012.30126121
AACGCAG220850.012.154922514
TGGTATC229700.012.0828137
ACGCAGA222800.012.06424715
CAGAGTA225950.011.834118
GCAGAGT227800.011.79944817
GTATCAA237450.011.4525739
Produced by FastQC (version 0.11.5)