FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n01_Rnf149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n01_Rnf149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29673456
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT359860.12127336970793022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT166800.016.00973920
GCAGTGG231650.015.6117872
GTACGGG89300.015.44189321
CGCAGAG232150.015.21171815
AGAGTAC233900.015.09790618
AGTGGTA243350.014.8319914
GTGGTAT246350.014.5945275
AGTACGG94750.014.55367820
GGTATCA246300.014.3947127
AGCAGTG250800.014.22829151
TACGGGG60900.014.19500222
GAGTACG99350.014.16165419
TCAACGC257450.013.74402811
ACGCAGA270650.013.13836914
TGGTATC274250.013.1097976
AACGCAG270550.013.03973513
CAGAGTA276400.013.00433517
GCAGAGT274400.012.94606316
GTATCAA282400.012.6413078
CAGTGGT299500.012.3201483
Produced by FastQC (version 0.11.5)