FastQCFastQC Report
Wed 15 May 2019
HKTFHAFXY_n01_Rnf149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTFHAFXY_n01_Rnf149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26390617
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT372550.14116759755939015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGG199250.017.6406783
AGCAGTG210700.016.6658632
GTGGTAT223300.015.6623416
AGTGGTA224650.015.6149725
GAGTACT187750.014.85707120
GGTATCA238400.014.680798
CGCAGAG250250.013.81776116
AGAGTAC252400.013.783257519
CAGTGGT262950.013.5802254
TGGTATC261700.013.3738637
TCAACGC262000.013.22481412
GTATCAA277900.012.6444759
AACGCAG278750.012.44267114
ACGCAGA282600.012.23600415
GCAGAGT283800.012.20893117
CAGAGTA289550.012.01483118
ATCAACG298350.011.82469911
CAACGCA296750.011.79410413
AGTACTT242100.011.65185521
GTACTTT265700.010.65643122
Produced by FastQC (version 0.11.5)