FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158446
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178430.4290785548255286No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG50110.12050174512305799No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG198850.049.1562570
CGGATCA74550.040.18749256
CCGGATC74050.040.17525555
TCTCGGG42500.039.52578743
CTCGGGG44900.039.43911444
GGATCAT66800.039.08653357
CGCCCGG14900.038.523252
CCGTATC352350.038.29260355
CGTATCA353600.038.2364256
GTATCAT338850.038.000357
TCGCCGG105350.037.9066952
TTCGCGA2600.037.7524031
CGCCGGA90250.037.69500453
CCCGGTC6200.037.25778254
TCATTAA441650.037.22876760
GTCGCCG485350.037.10768551
CGCCGTA376250.037.10669753
CATTAAA456000.036.82426561
TCGCCGT378200.036.72103552
GCCGGAT98650.036.72045554