FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4466825
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203210.4549316348860768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG109100.040.86790570
CCGGATC37100.034.43309855
CGTATCA180750.033.59528456
CCGTATC180350.033.5921755
CGGATCA39150.032.71948656
CGCCGTA192450.032.4257953
GTCGCCC40700.032.4175250
GTATCAT179350.032.41309757
CTCGGGG23700.032.1907644
TCGCCCG20550.032.0179451
TCGCCGT194100.031.9337752
CCCGATC10750.031.90617854
GTCGCCG249800.031.76190451
TCATTAA231100.031.51480960
CATTAAA236950.031.40211161
GCCGTAT202600.031.28554
TCGCCGG54800.031.23096852
GATCTCG251500.031.18441
TCTCGGG22900.031.02368443
CGCCCTA21550.031.02010552