FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161385
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132860.42025884224793875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG148450.045.66573770
TCTCGGG30950.036.4113143
CCCTATC23800.036.17536554
CCGGATC56200.036.11989655
CCCGGTC4950.036.05950554
CTCGGGG33600.035.83053644
CGAGATC36500.035.584395
CGGATCA57150.035.5807256
CCGTATC264950.035.32256755
GTATCAT254500.035.31522857
CGTATCA266500.035.0777356
TCATTAA327350.034.6284360
CATTAAA334900.034.47531561
GGATCAT52450.034.1648357
ATCATTA322750.033.99586559
CGCCGTA286350.033.7711153
TCGCCGT290200.033.21348652
GTCGCCG373700.033.20959551
GCCGTAT296100.033.10773554
CGCGTAG2650.033.0518462
Produced by FastQC (version 0.11.5)