FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681035
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG329180.8942593591204648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACC5500.057.954923
TCGTGAC6000.055.4801332
TTCGCGA3450.046.7347371
GTGACCT6750.046.6953434
CGGATCA135500.041.97542656
CCGAGAT13600.041.9743084
CGAGATC78800.041.9489945
TCGCGAG5650.041.551962
TCTCGGG119050.041.53640443
GGATCAT125000.041.4972457
TTCGTGA9950.041.215811
CCGGATC137150.041.2152355
TCATTAA609200.041.04873360
ATCATTA565150.040.81228359
CATTAAA626300.040.58738761
CTCGGGG128700.040.4613644
CGTATCA480750.040.377756
GCGAGAT28650.040.338914
CGCGAGA11550.040.3349273
GTATCAT463750.040.2125257
Produced by FastQC (version 0.11.5)