FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4468144
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174060.3895577224010685No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG48280.10805381384306324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG154200.043.2346270
CGCGAGA6900.036.550923
TCGCGAG3300.035.044062
CGAGATC41450.034.465165
CCCGGTC5100.034.31231754
CGGATCA62800.034.21833856
TCTCGGG36250.034.07868643
CCGGATC62600.034.04812255
CCGTATC287000.033.8888555
GTATCAT276500.033.75810657
CGGGTCA12150.033.7023256
TCATTAA349200.033.6952660
CGTATCA289200.033.6794656
GCGAGAT15750.033.352124
CATTAAA358500.033.31978661
CTCGGGG39450.032.8224444
ATCATTA349550.032.5401459
GGATCAT59350.032.31543757
CGCCGTA313000.031.82297553
CTAGATC35850.031.351665
Produced by FastQC (version 0.11.5)