FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4801801
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG317480.6611685907016971No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG443650.051.731970
CGCGAGA12750.044.2306373
TCTCGGG92400.044.20041343
CTCGGGG98950.043.67914244
CGGGTCA27250.043.6674656
TCGCGAG7450.043.28462
CCGGATC164700.043.00930855
CGCGTGA2450.042.941231
CGGATCA165550.042.6827856
TCGCCGG225050.042.4239352
CGCCGGG42000.042.41491353
CGCGCAA3850.041.9002341
CTCGCGA2850.041.8363151
GGATCAT147950.041.5389157
CGCCGGA201750.041.30436353
GCCGGAT211150.041.25526454
CTAGATC103000.040.7575845
GGGTCGC106100.040.67009449
CGAGATC110650.040.4711575
GCGAGAT37300.040.3708234
Produced by FastQC (version 0.11.5)