FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5240217
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142310.27157272303799634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA33050.029.6513253
CCGTATC33750.029.1400355
CTCGGGG10700.029.10949544
GTCGCCG46750.028.89686451
TCGCCGT34100.028.73775552
GGTCGCC49200.028.23983250
GCCGTAT37200.027.56653254
CGGCAAG15400.027.31221
GGGTCGC10200.027.10537149
ATCTCGG51800.026.88969642
TCTCGGG11550.026.6644943
CGCGAAG8550.026.64671
GATCTCG51750.026.64516841
TGGTCGC43250.025.8935949
CTCGGTG44350.025.48809244
CGTATCA39250.025.32422456
TCTCGGT45700.024.73539743
AGATCTC56600.024.300640
CGGGAAG27700.024.295041
CGCCGGA12850.023.96828753
Produced by FastQC (version 0.11.5)