FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7441097
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220240.2959778645541108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC71150.034.28717855
CGCCGTA70950.033.44653753
GTCGCCG94350.033.3481151
GGTCGCC98700.032.55056850
TCGCCGT73700.032.10355452
CGTATCA76700.031.7605356
ATCTCGG102950.031.71565842
GCCGTAT78000.031.63503854
GATCTCG103450.031.12238341
CTCGGTG93000.030.6685444
TGGTCGC92350.030.27807849
TCTCGGT94200.030.017843
TCGCCGG25800.029.4383852
GTGGTCG98000.028.85384848
AGATCTC112300.028.48313540
CCGGATC21400.028.45832655
GGGTCGC14950.028.32452249
CTCGGGG17700.028.27360544
CGCCGGA22450.028.06273753
TCTCGGG17550.027.31881743
Produced by FastQC (version 0.11.5)