FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6357060
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230040.36186539060509104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG119550.037.32288470
CGTATCA197250.031.40693356
CCGTATC196250.031.37078955
CCGGATC50000.030.17008455
CGCCGTA209250.029.87367653
CGGATCA51300.029.6102256
CATTAAA269150.029.43975661
GTATCAT205800.029.11572857
TCGCCGT216700.028.79773152
TCTCGGG35700.028.72240443
TCATTAA271500.028.70752360
CTAGATC30450.028.6340625
CGGGTCA14650.028.43011556
GCCGTAT224500.028.31188454
GTCGCCG295300.028.21942351
GATCTCG299150.028.16924741
ATCTCGG304550.028.0038942
AGATCTC299950.027.95543340
CTCGGGG38800.027.5994844
CTCGGTG283650.026.9821944
Produced by FastQC (version 0.11.5)