FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117438
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG187460.3663161136490564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG118500.040.81295870
CCGGATC42250.037.36019555
CCGTATC194450.036.28623655
CGTATCA196100.036.0880156
CGGATCA43600.036.04285456
CGCCGTA208450.034.94121653
CGAGATC29900.034.8955425
CTAGATC29700.034.7768635
TCGCCGT211100.034.4027752
GTCGCCG274700.034.36191651
CGCGCAG1950.034.16131
TCGCCGG62150.034.0141352
GATCTCG274750.033.82974641
GCCGTAT220800.033.5092954
ATCTCGG281400.033.50351342
AGATCTC279950.033.3645340
TCTCGGG27350.033.268843
CTCGGGG28650.033.22503344
GTATCAT205150.033.0117757
CTCGGTG263150.032.99472444
Produced by FastQC (version 0.11.5)