FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6633136
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221170.33343202973676406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG156850.041.78927270
CCCGGTC4850.037.5262754
CCGGATC51950.037.32484455
CGGATCA52800.037.05569556
CGCGGCC2950.036.7759966
TCTCGGG31850.036.03954343
CGTATCA259250.035.9525356
CCGTATC258800.035.8524855
TCGCCGG75600.034.90760852
GTCGCCG349700.034.8490251
CGCCCGG10750.034.8374152
CGCCGTA273450.034.80233453
CTCGGGG35000.034.7954844
GATCTCG343200.034.5666641
GGGTCGC36600.034.42279449
TCGCCGT275150.034.3705452
ATCTCGG353350.034.28706442
AGATCTC349000.034.02305640
TCTCGGT329600.033.49862743
GCCGTAT291700.033.4526254
Produced by FastQC (version 0.11.5)