FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683644
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136300.3700140404447335No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG38030.10324016110134422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG69400.036.9145470
CCGTATC115600.032.3638255
CGTATCA117350.031.97066156
CGCCGTA125550.030.85821553
TCGCCGT128700.030.18452552
GTATCAT120600.030.0353757
GTCGCCG163250.030.01336951
CCGGATC21750.029.92909655
CATTAAA151300.029.63085661
CGAGATC17650.029.5572645
GATCTCG162950.029.4879341
TCATTAA149400.029.46890860
CGGGTCA5250.029.33143456
GCCGTAT134250.029.3277154
ATCTCGG166050.029.23248342
AGATCTC165250.029.16301340
CGGATCA22950.028.97415756
TCTCGGG14850.028.51589843
CTCGGTG156450.028.45414544
TCGCCGG34500.028.40395752
Produced by FastQC (version 0.11.5)