FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300337
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125790.3811428954073478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTC2700.041.4813354
AAAGGGG89950.041.0460870
CCGGATC33350.034.63255755
CCGGTCA3100.033.87084655
CTAGATC20800.033.832245
CGGATCA34150.033.82125556
CCGTATC156300.033.7682755
CGTATCA156800.033.61594856
TCTCGGG21200.032.52100443
TCGCGAG2050.032.4792632
CGCCGTA167800.032.43432653
GTATCAT156200.032.37824257
CATTAAA203350.032.0475961
TCATTAA200800.031.6871760
TCGCCGT169800.031.68079452
CGAGATC20800.031.6440895
GTCGCCG221200.031.29640451
CGCGCAG1800.031.164361
CGACCAG1357.2759576E-1231.164361
CTCGGGG23200.031.07417544
Produced by FastQC (version 0.11.5)