FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4737735
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG188660.3982071601725297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG87000.036.1220270
CTCGGGG24150.031.73558444
CCGTATC140850.031.65759555
CGTATCA141600.031.36633156
TCTCGGG23050.030.97333343
GTATCAT140350.030.29874657
CGCCGTA150950.030.14224453
CCCGGTC3400.029.85275854
TCGCCGT153050.029.75090452
GTCGCCG204000.029.268551
GATCTCG201550.029.1362241
CCGGATC35800.029.13389855
GCCGTAT160550.029.12470454
TCATTAA184550.029.07170760
GGGTCGC25150.028.94308749
CATTAAA189750.028.79175461
ATCTCGG207900.028.71763442
CGGATCA36400.028.46136356
AGATCTC209250.028.16441340
CTCGGTG191450.027.93106744
Produced by FastQC (version 0.11.5)