FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5382493
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222110.4126526499895123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG175850.042.26950570
CCCGGTC6500.033.922454
CGCGGCC2600.033.6509966
CGCGAAA8300.033.3777121
TCTCGGG46600.033.11919843
CTCGGGG50950.032.62604544
GGGTCGC53550.032.15293549
CGTATCA320600.032.13909556
CCGTATC320150.032.0640255
CGCGCAA4050.032.0371781
TGTCGTG22450.031.8655471
CTAGATC40250.031.8417245
CATTAAA409350.031.69356261
CGGATCA78700.031.35261556
GTATCAT315600.031.27314257
TCATTAA406800.031.26419460
CCGGATC78700.031.26367255
CGGGTCA14950.031.13650556
CGCCGTA348700.030.19154253
TCGCCGT352200.029.89095552
Produced by FastQC (version 0.11.5)