FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5515969
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208320.37766709711385255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG247350.040.5495770
TCTCGGG83250.036.95002743
CTCGGGG88350.036.04532644
CCGTATC364950.035.49455
CGTATCA367000.035.37203256
TCATTAA480300.035.1074960
CATTAAA491150.035.0364361
CTAGATC51950.034.7810065
GTATCAT357700.034.71633557
CCGGATC110800.034.715855
CGGATCA110900.034.52669556
ATCATTA454750.034.47194359
CGAGATC59500.034.369555
CGCCGTA376200.034.3308653
GGGTCGC78550.034.1726849
CGCCCGG15100.034.0726352
GATCTCG543100.033.86739341
TCGCCGT385600.033.73811752
CCCGGTC9550.033.7173754
ATCTCGG554050.033.60891342
Produced by FastQC (version 0.11.5)