FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3917148
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196680.502099997242892No Hit
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG41070.1048466894791823Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG196000.043.02947670
CCGAGAT9900.039.266314
CGCGAGA7400.037.8734473
CGAGATC53250.037.7453585
CGCGGCC1151.8189894E-1236.5169166
CTAGATC39150.036.492175
GTCCGAG1350.036.3429952
CGCGCAG1950.035.9569551
CGCTAGA7900.035.919853
TGTCGTG20700.035.7354771
GCGAGAT19900.035.197294
TCGCGTA3600.035.058031
TCTCGGG57300.034.69000243
CCCCCTA4050.034.5695152
CAAGATC67500.034.4457445
CATTAAA423700.034.37854461
TCATTAA415450.034.35404660
GTATCAT315050.034.307757
CGTATCA333150.033.97770356
CCGTATC334500.033.861555
Produced by FastQC (version 0.11.5)