FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6575232
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172560.2624394089820709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA66000.029.21920653
CCGTATC66300.028.61188555
GTCGCCG89950.028.09227251
TCGCCGT68900.027.379852
GCCGTAT73000.026.70502954
GGTCGCC97450.026.6115850
CGTATCA71950.026.6083156
ATCTCGG99550.026.26034542
GATCTCG99500.025.88645241
CTCGGGG14150.025.72179644
TCGCCGG23800.025.58780552
CGCCGGA19900.025.50208353
CTCGGTG90850.025.50101544
TCTCGGT91150.025.18652743
TGGTCGC91950.025.12039849
CGGCAAG18250.024.978011
CGGGCAG8750.024.445511
TCTCGGG14100.024.32360843
CGCGAAG10800.024.0262131
GTGGTCG97900.023.91522248
Produced by FastQC (version 0.11.5)