FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6417035
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204670.3189479253268839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC59250.028.23658255
CGCCGTA59150.027.92928753
CGTATCA62400.027.25990156
TCGCCGT62900.026.37506352
GCCGTAT66550.025.50740254
GTCGCCG89650.024.9855451
GGTCGCC93500.024.3671550
ATCTCGG99700.023.79905742
TCTCGGG16250.023.69015743
GTATCAT69450.023.58537757
GATCTCG100200.023.26099841
CTCGGGG18050.023.07197844
TGGTCGC85000.022.97420549
CTCGGTG89200.022.91200344
TCTCGGT90600.022.44277243
CCGGATC21400.022.40677655
AGATCTC105600.021.67433540
TCATTAA106750.021.08120360
GTGGTCG95200.020.62248248
CGGATCA23600.020.61462256
Produced by FastQC (version 0.11.5)