FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739852
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120370.4393302995928247No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG35530.12967853738085122No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC31280.11416675061280682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC11350.029.29162630
CCGTATC11900.028.53014455
CGTATCA12150.028.23117456
CCGGATC7200.027.70904255
CGCCGTA10850.026.77487853
CGGATCA7250.026.55240256
ATCTCGG18600.025.96461542
TCGCCGT14350.025.60994352
GTGGGAC13050.025.20815319
TCGCGTC851.294423E-424.7485261
GATCTCG19550.024.523941
CGCGTAG1301.590015E-724.2610592
TGTCGTG7900.023.9653431
GAATGTC13850.023.7516527
CGGCGGC14000.023.74713733
GTCGCCG26300.023.68752351
CCGTGTC4900.023.57203155
GGTCGCC26450.023.42043750
CGGGCAG3050.022.9904331
GCCGTAT16400.022.83594954
Produced by FastQC (version 0.11.5)