FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_vk9-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_vk9-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4111007
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162970.39642355267213114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG294000.047.7817770
CGCGCAG2900.045.9309121
TCGCGAG4850.045.51222
CGCGAGA10050.043.5578843
TTCGCGA3500.042.0630461
CCCGGGC4300.041.51123454
CCCGGTC9550.040.31374754
CTAGATC63150.040.24845
CGGGTCA25700.039.76615556
GCGAGAT26150.039.633124
CGAGATC77300.039.4479145
CCGGTCA9900.039.2420555
TCTCGGG78350.038.36905343
CTCGGGG84000.037.78854844
CGCTAGA12150.037.758773
GTATCAT486750.037.6853157
GGATCAT109500.037.5886857
TCATTAA633900.037.46126660
GCGCAGA8800.037.426072
CGTATCA516700.037.26885256
Produced by FastQC (version 0.11.5)