FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10500041
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3539163.3706154099779226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG22900.041.77517343
CCGTATC97500.039.840855
CGTATCA99650.039.6472656
CTCGGGG27600.038.94532444
CGCCGTA103450.038.48373453
GATCTCG135400.038.25081341
GTATCAT96600.038.07843857
TCGCCGT102850.037.78931452
GCCGTAT110900.037.62966554
CCGGATC28600.037.6194655
AGATCTC138100.037.02078240
TCATTAA135500.036.6204660
TCGCCGG40550.036.40666252
TCTCGGT124250.036.32541743
CTCGGTG125800.036.23734744
AAAGGGG83150.036.15949670
TAGATCT147750.036.0029239
CGGATCA30300.035.96994856
GGATCAT28000.033.93428857
CGAGATC22600.033.8726435
Produced by FastQC (version 0.11.5)