FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158446
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60970.14661727000903704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGC8800.039.3745161
GCCGTGG88650.037.11178256
CCGTGGT87700.036.83534657
GGGTGGA67750.036.83349266
CGTGGTG74050.036.77199658
GGAAAAA309250.036.4651970
GAAAAAA419750.036.3295170
TCGGCGT103150.036.2041163
GCGTGGA118650.036.01725466
TGCCGTG130100.035.7259255
TCGTCGG64000.035.4917560
GTGGAAA192100.035.4732768
GTCGGCG59400.035.47094362
CGTGGAA123000.034.9995767
AAAGATC87900.034.7640085
GGCGTGA55100.034.74548765
TGGGGGT78750.034.6217963
ACACTCT31050.034.617951
GCCGTGT40650.034.61211856
CGGCGTG107950.034.4970364
Produced by FastQC (version 0.11.5)