FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4466825
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59740.13374152781897658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAA154150.036.7363470
GGAAAAA97950.034.3026570
TGGCGGG55500.031.15264155
ACACTCT32400.030.6915651
TGCCGGG9950.029.54725355
GGTGGCG48800.028.3294553
GCGGGGT34200.028.14281357
GGGGAAA190450.027.97013769
GGGTGGC52000.027.86492352
GTGGCGG50850.027.2561954
CGGAGTC5650.027.256197
GAGATCG113150.026.7287376
CGGGGTT32250.026.04608349
ATCGGGG322200.025.97256947
GGGGGAA201700.025.75069268
AATCGGG332150.025.59494246
ACGAGTG5750.025.56479638
CGGACAG3150.025.55513648
CGGGGGT118250.025.48372549
GAATCGG339900.025.1658145
Produced by FastQC (version 0.11.5)