FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161385
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53130.16805925251116205No Hit
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG33610.10631416293807934Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT37600.046.3706321
GGAAAAA126600.044.0951870
GGGAAAA216300.038.43007770
TGCCGGG20350.036.1176155
TCGGGGT46550.033.98465348
CGGGGTT41700.033.90856649
CCGGGGT8800.033.8065157
GGTTGGC29200.033.80106452
CGCGAGA6650.033.6860353
GTTGGCG26450.033.4779653
TTGGCGG29300.033.2078954
GTGAGAT16350.032.968154
TCCCGAA6500.032.84585615
AAAGATC62600.032.4857865
TTGCCGG14800.031.68890454
TGGCGGG95800.031.56547755
GCGTGAG3350.031.3449842
TGAGATC45450.030.5737155
GGTGGCG69500.029.96375753
GTTGCCG13000.029.61511853
Produced by FastQC (version 0.11.5)