FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681035
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60810.165198103250852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACG6100.054.50732812
AGGTCAC7700.043.1811311
GTGAGGT6150.041.54405642
GGAAAAA559950.041.2842870
GCCGTGG112800.040.98782356
CTCGGGT34900.040.615449
GAAAAAA351800.040.45131770
TCTCGGG42850.040.43109548
GAAGATC106950.040.419835
AAAGATC131800.040.394495
CCGTGGT118200.040.29969857
CCGGGGT136700.040.12006457
CGTGGTG123700.039.8093958
GTGAGAT31900.039.392374
GGTCGGC18700.039.30418861
TGCCGGG162350.039.1709255
ATCTCGG91350.038.84995747
GGGTGGA160650.038.80112566
TGCCGTG161750.038.58053255
CGGGTGC31350.038.40449551