FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4468144
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73520.16454259307667793No Hit
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG54250.12141506630045944Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT67000.048.0276641
GGAAAAA197200.042.8441570
GGGTGGA43350.037.05823566
CCGGGGT39600.036.1484257
TGCCGGG53600.036.04420555
TTGCCGG27650.035.56905754
GGTTGCC24050.033.7624652
GTTGCCG22800.033.1573753
GGGAAAA253250.032.96097670
GTGGAAA62250.032.04768868
GGTGGAA52700.031.41316467
AAAGATC76950.030.4776025
CGGGGTG142900.030.46837658
GCGGTTG24100.030.20699350
GTTGGCG35750.029.66386453
GCGTGGA11100.029.3238666
TTGGCGG41900.028.65109654
GCGTGAG5950.028.23992
GGTTGGC40050.027.87721352
CGTGAGA10550.027.8702873
Produced by FastQC (version 0.11.5)